Construction and Protection of Forest Resources
Liubin LI, Wen FANG, Ling MA, Yibo AN, Chao PAN, Lihong XIAN, Zhi DONG, Dan WEI, Xingzheng XIONG
Pine wilt disease, as a world quarantine disease, seriously endangers pine plants and threatens forest ecological security. However, there are currently no effective measures to control and eliminate it. To suit people's needs for supplying diverse ecological services to the forest, high-intensity (60% logging intensity) and low-intensity (15% logging intensity) thinning measures were used in Pinus massoniana forests to harvest wood infected with pine wilt disease. The alterations in bacterial and fungal composition in the soil of P. massoniana forests impacted by the disease were analyzed using high-throughput sequencing. The objective was to investigate the alterations in the organization of the soil microbial community in P. massoniana forests impacted by thinning-induced pine wilt disease and assess the effects on the soil microenvironment. The soil bacterial and fungal populations of P. massoniana woods were investigated using the high-throughput sequencing technique. The findings demonstrated that the bacterial and fungal populations in the soil varied significantly among the three stands. The high thinning measure forest group (H-CK) had the greatest Shannon diversity index and Chao1 index, and the diversity and richness of the soil microbial community in the H-CK group were considerably higher than those in the low thinning measure forest group (L-CK) and control group (CK). Actinobacteriota was the marker bacterial community and Leotiomycetes was the marker fungus community in the L-CK group. In the H-CK group, Chloroflexi was the marker bacterial community and Dothideomycotes was the marker fungal community. The high-intensity thinning H-CK group's soil organic matter content rose by 13% as compared to the control group, according to an analysis of soil physical and chemical properties. The H-CK group's total nitrogen content increased to 1.12 g/kg and its alkaline nitrogen content was 64.15 mg/kg as soil organic matter buildup increased. The forest's soil fertility was enhanced as a result, with a significant increase in both the total and accessible nitrogen content of the soil (P<0.05). Steadily impoving forest quality, strengthening the disease resistance and boosting the ecological function of the current, pure Pinus massoniana forest that has been harmed by pine wilt disease by progressively transforming it into a multilayer, unevenly aged needle broad-leaved mixed forest.